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HIV 1/2/O Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of antibodies to Human Immunodeficiency Virus(HIV) type 1,type 2 and subtype O in whole blood, serum or plasma to aid in the diagnosis of HIV infection.
Most popular quantity. HIV 1/2/O Rapid Test CassetteWhat is this product?HIV 1/2 test - is a rapid test for the diagnosis of Human Immunodeficiency Virus to detect antibodies to HIV type 1, type 2 and Subtype O qualitatively in whole blood, serum or plasma to aid in the diagnosis of HIV infection. It is intended for professional in vitro diagnostic use only. What should I know about HIV?
How this test works?The HIV 1/2/O Rapid Test Cassette(Whole Blood/Serum/Plasma) is a qualitative, membrane based immunoassay for the detection of antibodies to HIV-1, HIV-2, and Subtype O in whole blood, serum or plasma. The membrane is pre-coated with recombinant HIV antigens in the test line regions, T1 and T2. The T1 test line is pre-coated with HIV-1 and Subtype O antigen and the T2 test line is precoated with HIV-2 antigen. During testing, the whole blood, serum or plasma specimen reacts with HIV antigen coated particles in the test strip. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with recombinant HIV antigen on the membrane in the test line region. What should I remember using this product?
What should I know about kit storageand stability?
How should I use this kit?Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible.Place the cassette on a clean and level surface. For Serum or Plasma specimen: Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 25μL) to the specimen area, then add 1 drop of buffer (approximately 40μL),and start the timer, see illustration below. For Whole Blood specimen: Hold the dropper vertically and transfer 2 drops of whole blood (approximately 50μL) to the specimen area, then add 2 drops of buffer (approximately 80μL), and start the timer. See illustration below. Wait for the colored line(s) to appear. Read results at 10 minutes. Do not interpret the result after 20 minutes. INTERPRETATION OF RESULTS
POSITIVE:Two or three distinct colored lines appear. One line should always appear in the control line region, and another one or two apparent colored line(s) should appear in the test line region(s) (T1 and/or T2). *NOTE: The intensity of the color in the test line region (T1 and T2) will vary depending on the concentration of HIV antibodies present in the specimen. Therefore, any shade of color in the test line region (T1 and/or T2) should be considered positive. NEGATIVE: One colored line appears in the control region. No apparent colored lines appear in the test line regions (T1 and T2). INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test device. If the problem persists, discontinue using the test kit immediately and contact your local distributor. QUALITY CONTROLTo serve as a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred. A procedural control is included in the test. A colored line appearing in the control line region is considered an internal procedural control. It confirms sufficient specimen volume, adequate membrane wicking and correct procedural technique. |
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